Scopoletin (Synonyms: 东莨菪内酯; Gelseminic acid; Chrysatropic acid)

目录号: HY-N0342 纯度: 99.89%: Data Sheet SDS COA 产品使用指南
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Scopoletin 是一种乙酰胆碱酯酶 (AChE) 抑制剂。

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Scopoletin Chemical Structure

CAS No. : 92-61-5

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥393	In-stock
100 mg	￥358	In-stock
200 mg		询价
500 mg		询价

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Customer Review

Other Forms of Scopoletin:

Scopoletin (Standard) In-stock

MCE 顾客使用本产品发表的 3 篇科研文献

Scopoletin 相关产品

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AChE BChE

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Scopoletin is an inhibitor of acetylcholinesterase (AChE).

IC₅₀ & Target

AChE

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-431	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human A431 cells after 24 hrs by MTT assay Cytotoxicity against human A431 cells after 24 hrs by MTT assay	[PMID: 23218780]
A549	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 23218780]
A549	IC₅₀	> 20 μg/mL Compound: 14	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 23511021]
A549	IC₅₀	> 25 μM Compound: Scopoletin	Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
A549	IC₅₀	> 30 μM Compound: 6, Scopoletin	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
BXPC-3	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay	[PMID: 23218780]
DU-145	IC₅₀	> 50 μM Compound: scopoletin	Cytotoxicity against human DU145 Cytotoxicity against human DU145	[PMID: 16038556]
HCT-116	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay	[PMID: 23218780]
HCT-116	IC₅₀	> 25 μM Compound: Scopoletin	Growth inhibition of human HCT116 cells after 48 hrs by MTT assay Growth inhibition of human HCT116 cells after 48 hrs by MTT assay	[PMID: 27956344]
HCT-116	IC₅₀	> 30 μM Compound: 6, Scopoletin	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
HL-60	IC₅₀	9.5 μM Compound: 3	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 15387667]
HL-60	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HL60 cells after 24 hrs by MTT assay Cytotoxicity against human HL60 cells after 24 hrs by MTT assay	[PMID: 23218780]
HL-60	IC₅₀	> 50 μM Compound: scopoletin	Cytotoxicity against human HL60 Cytotoxicity against human HL60	[PMID: 16038556]
HT-1080	IC₅₀	12.5 μM Compound: 3	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 15387667]
HT-29	ED₅₀	> 10 μM Compound: scopoletin	Cytotoxicity against human HT-29 cells Cytotoxicity against human HT-29 cells	[PMID: 23301897]
HT-29	IC₅₀	> 100 μM Compound: 7, scopoletin	Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
HUVEC	ED₅₀	> 5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against HUVEC Cytotoxicity against HUVEC	[PMID: 15043409]
HUVEC	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against human HUVEC Cytotoxicity against human HUVEC	[PMID: 15270571]
HUVEC	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against HUVEC Cytotoxicity against HUVEC	[PMID: 17190457]
HeLa	IC₅₀	> 80 μM Compound: Scopoletin	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
Hep 3B2	IC₅₀	> 13.23 μg/mL Compound: 14	Cytotoxicity against human Hep3B cells Cytotoxicity against human Hep3B cells	[PMID: 23511021]
HepG2	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 23218780]
HepG2	IC₅₀	> 20 μg/mL Compound: 14	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 23511021]
HepG2	IC₅₀	> 25 μM Compound: Scopoletin	Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
HepG2	IC₅₀	> 50 μM Compound: scopoletin	Cytotoxicity against human HepG2 Cytotoxicity against human HepG2	[PMID: 16038556]
HuT78	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay	[PMID: 23218780]
Huh-7	IC₅₀	> 25 μM Compound: Scopoletin	Growth inhibition of human HuH7 cells after 48 hrs by MTT assay Growth inhibition of human HuH7 cells after 48 hrs by MTT assay	[PMID: 27956344]
K562	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human K562 cells after 24 hrs by MTT assay Cytotoxicity against human K562 cells after 24 hrs by MTT assay	[PMID: 23218780]
KB	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 15270571]
KB	IC₅₀	4 μg/mL Compound: Scopoltin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 9214732]
L02	IC₅₀	> 25 μM Compound: Scopoletin	Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 32438251]
L6	IC₅₀	> 90 μM Compound: 4	Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry	[PMID: 21438586]
LNCaP	ED₅₀	> 5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 15043409]
LNCaP	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 15270571]
LNCaP	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 17190457]
LoVo	IC₅₀	18.5 μM Compound: 3	Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay	[PMID: 15387667]
LoVo	IC₅₀	7.3 μM Compound: 3	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 15387667]
Lu1	ED₅₀	> 5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 15043409]
Lu1	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 15270571]
Lu1	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 17190457]
MCF-10A	IC₅₀	> 25 μM Compound: Scopoletin	Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 34315044]
MCF7	ED₅₀	> 5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 15043409]
MCF7	ED₅₀	> 5 μg/mL Compound: scopoletin	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 17190457]
MCF7	IC₅₀	17.11 μg/mL Compound: 14	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 23511021]
MCF7	IC₅₀	> 100 μM Compound: 7, scopoletin	Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
MCF7	IC₅₀	> 25 μM Compound: Scopoletin	Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
MCF7	IC₅₀	> 25 μM Compound: Scopoletin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 34315044]
MCF7	IC₅₀	> 50 μM Compound: scopoletin	Cytotoxicity against human MCF7 Cytotoxicity against human MCF7	[PMID: 16038556]
MCF7	IC₅₀	> 80 μM Compound: Scopoletin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
MDA-MB-231	IC₅₀	> 100 μM Compound: 7, scopoletin	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
MDA-MB-231	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay	[PMID: 23218780]
MDA-MB-231	IC₅₀	> 20 μg/mL Compound: 14	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 23511021]
MDA-MB-231	IC₅₀	> 25 μM Compound: Scopoletin	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 34315044]
MDA-MB-231	IC₅₀	> 30 μM Compound: 6, Scopoletin	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
MDA-MB-435	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay	[PMID: 23218780]
MGC-803	IC₅₀	> 80 μM Compound: Scopoletin	Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
MKN-45	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay	[PMID: 23218780]
Macrophage	IC₅₀	5 μM Compound: 4, scopoletin	Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method	[PMID: 8988605]
NCI-H460	IC₅₀	> 50 μM Compound: scopoletin	Cytotoxicity against human NCI-H460 Cytotoxicity against human NCI-H460	[PMID: 16038556]
Neutrophil	IC₅₀	> 50 μM Compound: 11	Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release	[PMID: 18211005]
Neutrophil	IC₅₀	> 50 μM Compound: 11	Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation	[PMID: 18211005]
PANC-1	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay	[PMID: 23218780]
PBMC	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human PBMC after 24 hrs by MTT assay Cytotoxicity against human PBMC after 24 hrs by MTT assay	[PMID: 23218780]
PC-3	IC₅₀	72.19 μM Compound: Scopoletin	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
Platelet	IC₅₀	41.74 μg/mL Compound: Scopolatin	Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry	[PMID: 21106454]
Platelet	IC₅₀	> 50 μg/mL Compound: Scopolatin	Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry	[PMID: 21106454]
SK-MEL-28	IC₅₀	3.43 μM Compound: 2	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	1.5 μM Compound: 2	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SW-620	IC₅₀	> 25 μM Compound: Scopoletin	Growth inhibition of human SW620 cells after 48 hrs by MTT assay Growth inhibition of human SW620 cells after 48 hrs by MTT assay	[PMID: 27956344]
Sf21	IC₅₀	> 100 μM Compound: 7	Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay	[PMID: 17378609]
U-937	CC₅₀	600.2 μM Compound: 3	Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs	[PMID: 18060791]
U-937	CC₅₀	> 2000 μM Compound: 8	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]
U-937	IC₅₀	151.5 μM Compound: 3	Growth inhibition of human U937 cells by [3H]thymidine incorporation assay Growth inhibition of human U937 cells by [3H]thymidine incorporation assay	[PMID: 18060791]
U-937	IC₅₀	975.9 μM Compound: 8	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]

体外研究
(In Vitro)

Scopoletin (SCT) 被确定为乙酰胆碱酯酶 (AChE) 的推定抑制剂。Scopoletin 增强 K⁺ 刺激的大鼠额叶皮质突触体 ACh 释放，显示出钟形剂量效应曲线 (E_max：4 μM)^[1]。Scopoletin 通过诱导 PC3 细胞凋亡来抑制 PC3 增殖。Scopoletin抑制PC3、PAA (人肺癌细胞)、Hela 细胞增殖的 IC₅₀ 分别为 (157±25)、(154±51)、(294±100) mg/L。Scopoletin 诱导显著的时间和浓度依赖性 PC3 细胞增殖抑制。Scopoletin 以浓度依赖性方式降低 PC3 细胞中的蛋白质含量和酸性磷酸酶活性 (ACP) 水平。光镜、荧光显微镜、透射电镜观察 Scopoletin 处理后的细胞，显示出典型的细胞凋亡形态学变化。Scopoletin 0、100、200、400 mg/L 时细胞凋亡率分别为 0.3%、2.1%、9.3%、35%，东莨菪素处理后 G2 期细胞明显减少^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Scopoletin (2 μg，icv) 增加 T 迷宫交替并改善东莨菪碱诱导的胆碱能缺陷小鼠的新物体识别。 Scopoletin 还可以减少 15-18 个月大的小鼠（2 mg/kg sc）的对象记忆中与年龄相关的缺陷。注射 2 μg Scopoletin 的小鼠表现出 71.3±2.5% 的交替率增加^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

192.17

Formula

C₁₀H₈O₄

CAS 号

92-61-5

性状

固体

颜色

White to yellow

中文名称

东莨菪内酯；莨菪亭；东莨菪亭

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

细胞实验：

DMSO 中的溶解度 : 62.5 mg/mL (325.23 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

Ethanol 中的溶解度 : < 1 mg/mL (insoluble)

*Scopoletin is usually formulated as a suspension.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.2037 mL	26.0186 mL	52.0373 mL
5 mM	1.0407 mL	5.2037 mL	10.4075 mL
10 mM	0.5204 mL	2.6019 mL	5.2037 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.82 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (10.82 mM); 悬浊液; 超声助溶

此方案可获得 2.08 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (10.82 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.89%

选择批次：

Data Sheet (651 KB) SDS (393 KB)

COA (286 KB) LCMS (94 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Hornick A, et al. The coumarin Scopoletin potentiates acetylcholine release from synaptosomes, amplifies hippocampal long-term potentiation and ameliorates anticholinergic- and age-impaired memory. Neuroscience. 2011 Dec 1;197:280-92. [Content Brief]

[2]. Liu XL, et al. Effect of Scopoletin on PC3 cell proliferation and apoptosis. Acta Pharmacol Sin. 2001 Oct;22(10):929-33. [Content Brief]

Cell Assay ^[2]	PC3 cells (5×10⁷/L) 1 mL in exponential growth are seeded into four 24-well plates. The plates are incubated at 37°C in a humidified 5% CO₂ atmospbee. After 24h, Scopoletin 33, 66, 133, 266, and 533 mg/L are added to wells (3 wells for each concentration for each plate). For control cells (3 wells foreach plate),only DMEM was added. The plates are incubated continually. The viable cells are counted by hemocytometer every day in the frist 4 d by Trypan blue dye exclusion method^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] C56BL/6N male, 4-6-month-old and 16-18-month-old mice are used in the behavioral studies. The mice are housed in groups of four in cages at constant humidity (50-55%) and temperature (22±1°C) on a 12:12 h light/dark cycle (7:00–19:00 h), with food and water ad libitum. Younger mice (4-6 months) are implanted with i.c.v. cannulas for application of Scopolamine (SCOP) and Scopoletin. The aged mice are injected with Scopoletin by the s.c. route. Experiments are conducted between 8:00 and 16:00 h. Mice with i.c.v. cannulas are randomly divided into four experimental groups: vehicle; SCOP 20 μg; Scopoletin 2 μg; and SCOP 20 μg plus Scopoletin 2 μg. The drugs are applied in 1 μL of vehicle solution (SCOP: saline, Scopoletin: 3 DMSO: 7 sterile water). I.c.v. injections are carried out 15 min before the start of the tests. Aged mice obtained Scopoletin s.c. 30 min prior to object memory test (vehicle: 1 DMSO: 1 EtOH, diluted with olive oil as required)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Hornick A, et al. The coumarin Scopoletin potentiates acetylcholine release from synaptosomes, amplifies hippocampal long-term potentiation and ameliorates anticholinergic- and age-impaired memory. Neuroscience. 2011 Dec 1;197:280-92. [Content Brief] [2]. Liu XL, et al. Effect of Scopoletin on PC3 cell proliferation and apoptosis. Acta Pharmacol Sin. 2001 Oct;22(10):929-33. [Content Brief]

Scopoletin 相关分类

Inflammation/Immunology

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.2037 mL	26.0186 mL	52.0373 mL	130.0931 mL
	5 mM	1.0407 mL	5.2037 mL	10.4075 mL	26.0186 mL
	10 mM	0.5204 mL	2.6019 mL	5.2037 mL	13.0093 mL
	15 mM	0.3469 mL	1.7346 mL	3.4692 mL	8.6729 mL
	20 mM	0.2602 mL	1.3009 mL	2.6019 mL	6.5047 mL
	25 mM	0.2081 mL	1.0407 mL	2.0815 mL	5.2037 mL
	30 mM	0.1735 mL	0.8673 mL	1.7346 mL	4.3364 mL
	40 mM	0.1301 mL	0.6505 mL	1.3009 mL	3.2523 mL
	50 mM	0.1041 mL	0.5204 mL	1.0407 mL	2.6019 mL
	60 mM	0.0867 mL	0.4336 mL	0.8673 mL	2.1682 mL
	80 mM	0.0650 mL	0.3252 mL	0.6505 mL	1.6262 mL
	100 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3009 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Scopoletin92-61-5Gelseminic acid Chrysatropic acid东莨菪内酯莨菪亭东莨菪亭Cholinesterase (ChE)ApoptosisInhibitorinhibitorinhibit

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Scopoletin (Synonyms: 东莨菪内酯; Gelseminic acid; Chrysatropic acid)

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Scopoletin (Synonyms: 东莨菪内酯; Gelseminic acid; Chrysatropic acid)

Customer Review

Other Forms of Scopoletin:

Scopoletin 相关抗体

MCE 顾客使用本产品发表的 3 篇科研文献

Scopoletin 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 Cholinesterase (ChE) 亚型特异性产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Scopoletin 相关抗体:

摩尔计算器

稀释计算器

Scopoletin 相关分类

完整储备液配制表

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